FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-40_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-40_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707993
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTC325950.5710413450051532No Hit
GTGCAGGTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGA258570.45299635090652707No Hit
GGTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGAGGAGT251510.4406277302722691No Hit
CCATCAAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCG164720.28857778907577497No Hit
GTAGAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTCC137500.2408902743924178No Hit
GGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA120390.21091476461165948No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATA116090.20338146875793295No Hit
GTGTTAAATAACTAGGAATTTTTTAAAAAACAAACAAACAAAACAAAACA107530.18838495422121226No Hit
CCCAAAGAGAGATTACTAAGAGTTTCATTTAAAATCTTTTTTTTTTTTTT105120.1841628046845888No Hit
GTATTCGTAAGAATACATGTATTACAAGGAATTTCCCTATGTCTTAGGAT102310.1792398834406419No Hit
CCATAACCATCACCACCTCTACTACCACCTCCACCACCATCACCATAACC97870.17146131748935223No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCACCACCATA88740.15546620326969568No Hit
CCCTTTTGACAATGTATTCGTAAGAATACATGTATTACAAGGAATTTCCC85260.1493694894159821No Hit
ATTTTGTGCTGACATCCGGGTAAGACAGAACCACAGGAAACCCACGGGAA84240.14758252156230744No Hit
GTTTTAGGCATTATCCAAATCCAAACTGATTTGCAACACTTTTATGAGAG83950.14707446207449798No Hit
CCATCAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCGT83670.1465839218793716No Hit
GTTCCTAGGTAGTAGAAATGAGATCAGGCACCTGATTAAAGTAGCAGTCT81950.143570603537881No Hit
GGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGA81260.14236177234274813No Hit
GTAGAAATACTAGGTAAAAAACAGCAAACCAAACCAAACAGTAAGGAACA79470.13922581895247593No Hit
ATATCAACCTCATTCCCTTTTGACAATGTATTCGTAAGAATACATGTATT79290.13891047168418041No Hit
CCTCTACTACCACCTCCACCACCATCACCATAACCACCATCACCACCTCT77090.13505622729390174No Hit
GATACATGCAGTGTGGTCTCTGGACCTCTAAGCCTCTGCTTCATCAGGAA76080.13328677873291014No Hit
GATATTCCCTTGCTTTACAACAATGGGTTGGCCACCCAAAGAGAGATTAC74110.12983547807434243No Hit
CATCAAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCGT72290.12664696680602097No Hit
ACAATGGGTTGGCCACCCAAAGAGAGATTACTAAGAGTTTCATTTAAAAT69370.12153133334256015No Hit
GTTCCTAGGTAGTAGAAATAGAGGTTAAGGAGGGTGATGGTGGCTATGAT68730.12041009861084272No Hit
ATCCTAAGACATAGGGAAATTCCTTGTAATACATGTATTCTTACGAATAC68580.12014730922059644No Hit
GAATACATGTATTACAAGGAATTTCCCTATGTCTTAGGATCGAGTAACCC68350.1197443654888855No Hit
ATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTCCATGAGAAC66960.11730918380593669No Hit
GTTCCTAGGTAGTAGAAATACTAGGTAAAAAACAGCAAACCAAACCAAAC66790.11701135583032427No Hit
GGGTAAGACAGAACCACAGGAAACCCACGGGAACAGGTCCAAGTGGATGA64860.11363013234248887No Hit
GTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGAGGAGTT64470.11294687992784855No Hit
GTTCCTAGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTG64380.11278920629370077No Hit
TTATAAGGACTGTGGCATGGACTGGCTTTCCTTAACAGCACTGGAAGCTT63670.11154533651320175No Hit
GAATAGCTCAGTGTTAAATAACTAGGAATTTTTTAAAAAACAAACAAACA61200.10721807122047979No Hit
GGTAAGGGCTCCTGGGGGTCAAACGCATTTCAGCAAATCTTCCTGATGAA60550.10607931719607926No Hit
ATTTAACACTGAGCTATTCCTTTGTATGTAAACCTTCAATTAAAAGAAAA58400.102312669269216No Hit
GTTTACATACAAAGGAATAGCTCAGTGTTAAATAACTAGGAATTTTTTAA57920.10147174322042792No Hit
AATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTCCATGAGAACAGCAT57690.10106879948871696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA101650.0112.306351
AGGTATA51150.0102.302125
GCAGGTA59500.088.067243
TCCTAGG130800.087.325593
CCTAGGT133350.085.492654
TAGGTAG144900.078.826696
CAGGTAT67800.077.393044
GTAGCAA68500.072.376341
TGCAGGT77250.068.020552
TTCCTAG172800.066.227612
CTAGGTA175150.065.29655
GTGCAGG84000.062.904161
GGTATAT86600.060.5070346
CCGCTTA12250.060.341929
ATCAAGC45650.058.4251863
TGTAGAG71550.056.117822
TGCCGCT13400.055.70567
GCCGCTT13450.054.9582528
CAAATAT91950.054.071135
TAGCAAC42550.052.4665221
Produced by FastQC (version 0.11.5)