FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-39_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-39_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12206303
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT559560.45841890046478445No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG409620.33558072415538104No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC335110.2745384904831545No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA331110.2712614949833705No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC330090.27042586113092554No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT297030.24334149332521077No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA262640.21516752451581778No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC254680.20864630347124763No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA251870.20634421413264933No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA241750.1980534155181958No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA240550.1970703168682606No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG228060.18683789842018503No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT226950.185928532168995No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT225110.18442111423909432No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT224970.1843064193966019No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC222280.18210263992299716No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC221410.18138989340179412No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT219890.18014463511187623No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT216400.17728545653831468No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA212950.17445904791975098No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC211010.17286970510235572No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT210000.17204226373866024No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG204450.16749543248270995No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC201550.16511961074536655No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA192370.15759890607336227No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT192060.157344938922129No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC191820.15714831919214195No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT190600.15614883556470785No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG190340.15593583085722187No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA189950.15561632379599294No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC188420.15436287301732557No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA188290.15425637066358258No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC185970.15235571327370787No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC178910.1465718162165891No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA176330.1444581541192284No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG174870.14326205076180726No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA170830.13995228530702541No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG170180.13941977353831048No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG170130.1393788110945632No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT166020.13601169821853512No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC164940.13512690943359346No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT162720.13330817693121333No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC162380.1330296323137317No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC162270.13293951493748762No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG160100.1311617448788548No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG159090.13033430351515934No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG153780.12598409198919608No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA153120.12544338773173172No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT149320.12233024200693692No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG147740.12103582878452222No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA145960.11957756578711834No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG145430.11914336388339697No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC145220.1189713216196583No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT144520.1183978474071961No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT143920.1179062980822285No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT143440.11751305862225442No Hit
GTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGA143200.11731643889226738No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC140760.11531747163739915No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT140170.114834114801181No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG139500.11428521805496718No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC138370.11335946682627818No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA138250.11326115696128466No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT138120.11315465460754169No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT137920.1129908048325525No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT137650.11276960763631706No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA136550.11186843387387647No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG135350.11088533522394127No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA134670.11032824598897799No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC133250.10916491258655465No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC133170.10909937267655899No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG132960.10892733041282032No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC131010.10732979510667563No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA130800.10715775284293697No Hit
AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT129980.10648596876548125No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT129280.10591249455301904No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG128420.1052079405205655No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT128280.10509324567807304No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC125530.10284031127197153No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC124500.10199648493077716No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT123820.10143939569581388No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT123440.10112808112333439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAAT38050.0111.989932
TCAATGT39250.0108.380074
TCCCTAG27700.0108.050452
GAATATT17150.0102.267541
GACGAGG2350.0101.773021
CCCTAGC96400.0101.569871
CCTAGCA97700.0100.137222
AGGGTAG9850.0100.0584263
GTCTCAA14150.098.340261
AAGGGTA10450.093.621122
CTCCCTA34600.086.927781
ACGAGGT2800.085.410692
GGGCTCA2000.083.345931
GAAGGGT12000.081.534071
GGGTAGT33150.080.6732251
CGTCCGA300.001937998372.48364145
GAAGACA31200.072.004254
CGAGGTC3600.070.4557653
GCCCACC13800.067.2312145
TAGAGCC41650.066.987954
Produced by FastQC (version 0.11.5)