FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-39_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-39_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9629471
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA614590.6382385906764764No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT362020.37595003920776127No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC356000.3696983977624524No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC254250.2640331955929874No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA252220.2619250839428251No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG250180.2598065875062088No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA222350.2309057268047227No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA220690.22918185225335846No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT215440.2237298393650077No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC214740.2230029043132276No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC213630.22185019301683342No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT212200.2203651685539112No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG208720.2167512628679187No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG194300.20177640080124856No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT192860.20028099155187237No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC178250.18510881854257621No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG173810.18049797335699957No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT170280.17683214373873704No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA168640.1751290387602808No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC163970.1702793434862621No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG162350.1685970080807139No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC161890.1681193079038298No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG161070.16726775541460168No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT158250.16433924563457328No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT157390.16344615399952916No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA155030.1609953443963848No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG154930.16089149653184479No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG152490.15835760863706844No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA150360.15614564912236611No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA149600.155356405351862No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA149410.15515909440923598No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA149410.15515909440923598No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT145900.15151403436388144No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC145420.15101556461408938No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT141120.14655010643886876No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA140090.1454804734341066No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA139430.14479507752814252No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA139120.14447314914806847No Hit
TTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATT136960.14223003527400413No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC135940.141170787055696No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG134540.13971691695213578No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT134470.1396442234469578No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC132450.13754649658324947No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA131360.13641455485976334No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT129790.1347841433864851No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC129240.134212980131515No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT129070.134036438761797No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT128390.13333027328292488No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA127510.13241641207497276No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC122250.12695401440016799No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC119790.12439935693248363No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT117570.12209393433969529No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT117330.12184469946479927No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT115470.119913129184355No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG114240.11863580045051281No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG114070.11845925908079478No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT114040.11842810472143278No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA113690.11806463719554272No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG112450.11677692367524654No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA110040.1142741901398322No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG109200.11340186807769607No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT108530.11270608738527797No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT108060.1122180024219399No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA107880.11203107626576787No Hit
GTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGAC107100.11122106292235576No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC106330.11042143436539764No Hit
GATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACA105640.10970488410007155No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC105580.10964257538134753No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA104300.10831332271523533No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG104000.10800177912161531No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC102180.10611174798698703No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT101290.1051875019925809No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT100670.1045436452324328No Hit
CTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTAT100410.10427364078462877No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG99910.1037544014619287No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT98990.10279900110816056No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT98830.10263284452489654No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA98480.10226937699900648No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT98220.10199937255120245No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG97900.10166705938467441No Hit
CCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTT97790.10155282673368039No Hit
GTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTT97650.10140743972332437No Hit
GATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACATAACAGCAGT97210.1009505091193483No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA97110.10084666125480829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG32000.0111.8803563
TTCCTAG33350.0104.0950552
TAGGTAG35350.0101.893426
GTTCCTA36250.096.5751951
CCTAGGT38050.094.281714
CTAGGTA41250.087.670955
CAGGTAT15750.078.224944
CGGACTG651.5716068E-978.048365
AAATTAG149100.066.689225
AGGTAGT55150.065.705117
AGTCCCG1800.060.404293145
GAAGGAG7500.059.9114155
GCAGGTA20950.059.500593
CCGTTCA9050.058.468685145
GATCCGG502.0136735E-457.985111
AAGTCCG2400.057.38408145
GGCGTGT37000.056.214468
GATAGGC4900.056.2036363
ATAGGCG37000.056.0200425
TGCGCTA24550.055.4984667
Produced by FastQC (version 0.11.5)