FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-38_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-38_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10653276
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAA764870.7179669427507558No Hit
CCCTAGCATTCACCAATGACCTGAACGGTGAGCACCCGCACAAGGCAGCG761170.7144938326952198No Hit
ACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAA645400.6058230350926795No Hit
ATGTTAAATGGACTCACCTTGATTATGAGTTCCAGGTCTGTCAAGACACA576840.5414672444419913No Hit
CAATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTT545910.5124339217344975No Hit
GTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTT430280.40389453910703144No Hit
CTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGAAT401090.37649451680403284No Hit
GTCCAACACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAA349940.3284811169822316No Hit
AATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTC326230.30622505227499974No Hit
TTAAAGAAGTGTGTCTTGACAGACCTGGAACTCATAATCAAGGTGAGTCC325140.3052018928262067No Hit
ATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAG323570.3037281677485874No Hit
GTCTCAAGGGCTGTTGTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGT304020.28537700515785No Hit
CTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGAATTAACCTGCC294030.2759996080079029No Hit
CCCTAGCATTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAG257080.2413154413722126No Hit
GCATTCACCAATGACCTGAACGGTGAGCACCCGCACAAGGCAGCGGACAT256290.24057388544143607No Hit
GTCAAGACACACTTCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACT255490.23982294272672555No Hit
ATTTAACATTGTCCAAACTCCAAAAGTACTCTCAGCATGCTGCCTTGGCA254720.23910016036381676No Hit
GTCCAGTAAGGTCAGGAGGCATAGAACTCATGGTTAAAGTTTTGGAGATT252200.2367346908124787No Hit
GAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTTGGACA251280.2358711066905617No Hit
GTGCAGGAATCGCCGGCCTGTATCTCATAAGCATAAACATTCACAAACTC250250.23490426794537195No Hit
CTCAAGGGCTGTTGTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGTAA249070.23379662744117397No Hit
GTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAG241710.22688795446583754No Hit
CCAATGACCTGAACGGTGAGCACCCGCACAAGGCAGCGGACATGGAGGGT232150.2179141890250473No Hit
CCTGTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATA227720.21375584374233805No Hit
CATGGAGAAGACAAAGAACACACAGGAGAATTAACCTGCCAAGGCAGCAT226860.21294858032402425No Hit
GAGTATGCTCAGTGCAGGAATCGCCGGCCTGTATCTCATAAGCATAAACA226660.21276084464534667No Hit
GTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGACA218110.2047351443818784No Hit
GCATAGAACTCATGGTTAAAGTTTTGGAGATTTCTTTTTTTGCAAGTTTT213810.20069882729030958No Hit
GTATGTATGTGACTGGCAATATGTCAAATGCCTGTTGAGAGTTTGGAGAG213450.20036090306868987No Hit
GTCCTACATCCACAGTGCTGTCGATGGGTGAAAATTCCAAGTACTGCAGC212970.19991033743986358No Hit
GTTTTATAGTGAGCCTATGCACTGTGAACTTCACCAGTGTTGTGCAGGCT212440.19941283789136785No Hit
CCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGGTGC205680.19306737195206433No Hit
CTTTTGGAGTTTGGACAATGTTAAATGGACTCACCTTGATTATGAGTTCC203870.19136836406003188No Hit
GTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGG200330.188045442547438No Hit
GATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTTGAAAAG199660.18741652802386796No Hit
GTTTAGGCAGGATTCTGCCCTTGGAAACTGCTGGAATTTAACTAAAGATA196920.18484454922598456No Hit
CTTCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACTCTGTCCTACAT195330.1833520505804975No Hit
GAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGA191990.18021686474658125No Hit
GTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGTAATACCTAATCCCAG189650.1780203573060531No Hit
CCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGTGAA189000.17741021635035084No Hit
CTCCCTAGCATTCACCAATGACCTGAACGGTGAGCACCCGCACAAGGCAG187730.17621809479074793No Hit
GTATTTATGAAGAGAAGTCACCTTAAAGGCAGAGACTGCAGCATTTCTTT187240.17575814237798779No Hit
GGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTTGG186550.17511045428654998No Hit
CCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGA186490.1750541335829467No Hit
GTGTCTTGACAGACCTGGAACTCATAATCAAGGTGAGTCCATTTAACATT185890.17449092654691384No Hit
CAATGTGTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAA185040.17369304991253393No Hit
ATCAAGGTGAGTCCATTTAACATTGTCCAAACTCCAAAAGTACTCTCAGC183490.1722380984027824No Hit
CCTTAAAGGCAGAGACTGCAGCATTTCTTTTTGTCCTACCATTATTCTAG182030.17086762794843577No Hit
GGCTCAATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCA182020.17085824116450188No Hit
AGCACTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAGTGCA180920.169825694931775No Hit
GTTAAATGGACTCACCTTGATTATGAGTTCCAGGTCTGTCAAGACACACT180560.16948777071015528No Hit
CACCTTGATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTT179740.16871805442757704No Hit
TATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCT174510.16380876643015727No Hit
GTACAACATGCATGTTGTATGTATGTGACTGGCAATATGTCAAATGCCTG170240.1598006096903901No Hit
CTACAGTTCTGTGATTAGGTTTCAGTTTTATAGTGAGCCTATGCACTGTG168560.15822362998949807No Hit
TCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACTCTGTCCTACATCC167310.15705028199776294No Hit
CATACATACAACATGCATGTTGTACACTGCAACTCTTAAAAGAGCTGGGA163720.15368042656549966No Hit
GCATACTACAGTTCTGTGATTAGGTTTCAGTTTTATAGTGAGCCTATGCA160200.15037627862077355No Hit
CAGGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTT160060.15024486364569922No Hit
CCTTGATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTTGA158440.1487242046484105No Hit
GTTCATTGTATCCCTAACCTTAACCAGATCACCACAGAACTCTCCAAACT154700.145213547457139No Hit
ACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGT154450.144978877858792No Hit
GTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAA153500.14408713338507328No Hit
TAATCAAGGTGAGTCCATTTAACATTGTCCAAACTCCAAAAGTACTCTCA153370.14396510519393285No Hit
CTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACC153260.14386185057066014No Hit
CACCTGTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCA151070.1418061448891402No Hit
GTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTA151030.1417685977534047No Hit
CTCCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGTG147390.138351808401472No Hit
CCCTAGCATTCATCGCCATATCTTGCTTCTCTTTTTCCTTAGAGCTCCTT146590.13760086568676153No Hit
GCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAG144090.1352541697032913No Hit
GGAGAAGACAAAGAACACACAGGAGAATTAACCTGCCAAGGCAGCATGCT143920.1350945943764153No Hit
CTCCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGGT143770.1349537926174071No Hit
GTAATACCTAATCCCAGCTTCCTCTAGCCATCCTATCTTGCCTAAGGCAG139990.13140558829040005No Hit
GTACTTGGAATTTTCACCCATCGACAGCACTGTGGATGTAGGACAGAGTG139090.13056077773635077No Hit
GTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGACAGA138740.13023224029866493No Hit
TGTACACTCTGTCCTACATCCACAGTGCTGTCGATGGGTGAAAATTCCAA137990.12952823150362386No Hit
GAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACA136470.12810144034567397No Hit
CAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACAC135820.1274912993899717No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCT134530.12628040426250103No Hit
GTGTACAACATGCATGTTGTATGTATGTGACTGGCAATATGTCAAATGCC134210.12598002717661685No Hit
CAGTAAGGTCAGGAGGCATAGAACTCATGGTTAAAGTTTTGGAGATTTCT134120.12589554612121193No Hit
CCTATGCACTGTGAACTTCACCAGTGTTGTGCAGGCTCCTCCCTGCCTTA133610.12541682014058397No Hit
ACCTTAACCAGATCACCACAGAACTCTCCAAACTCTCAACAGGCATTTGA132850.12470342456160903No Hit
CCACAGAACTCTCCAAACTCTCAACAGGCATTTGACATATTGCCAGTCAC131860.12377413295215482No Hit
CAGCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCAT130190.1222065400351967No Hit
CCATTATTCTAGTTATTTCCTGATTCCCAGCTCTTTTAAGAGTTGCAGTG129950.12198125722078354No Hit
GTATCTCATAAGCATAAACATTCACAAACTCACACAGTTTAGGCAGGATT129700.12174658762243652No Hit
CGACAGCACTGTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTC129630.12168088013489935No Hit
TCCAGGCACAGCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTT128620.12073281495757737No Hit
CAATGTGTCCAGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGT127650.11982229691599092No Hit
ACTTGGAATTTTCACCCATCGACAGCACTGTGGATGTAGGACAGAGTGTA127030.1192403163120903No Hit
GCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCATCG125360.11767272339513217No Hit
AAGATACTCTGTTCATTGTATCCCTAACCTTAACCAGATCACCACAGAAC123620.11603942299063685No Hit
GGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACCTT122760.11523215957232312No Hit
GTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTG119580.11224716228134894No Hit
GCACTGTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCA118650.11137419137549802No Hit
CTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAGTT118160.11091423896273786No Hit
GCATTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAGTGCAG117880.11065140901258919No Hit
GGCTCAATGTGTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGG117520.11031348479096947No Hit
AATGAGAACTGCTGGGGGAGCAGCAGCATACTACAGTTCTGTGATTAGGT116640.10948744780478793No Hit
GTGCATAGGCTCACTATAAAACTGAAACCTAATCACAGAACTGTAGTATG116100.10898056147235835No Hit
GGTTAAGGTTAGGGATACAATGAACAGAGTATCTTTAGTTAAATTCCAGC115260.10819207162191236No Hit
ACCTAATCCCAGCTTCCTCTAGCCATCCTATCTTGCCTAAGGCAGGGAGG114270.10726278001245815No Hit
CCCTTGTATTTATGAAGAGAAGTCACCTTAAAGGCAGAGACTGCAGCATT113760.10678405403183021No Hit
GTTGTACACTGCAACTCTTAAAAGAGCTGGGAATCAGGAAATAACTAGAA113630.10666202584068976No Hit
CCTTAAGGACAGGAGCCATTTCTTATTAATGCTTGTCAACATAGCACTTG113330.10638042232267333No Hit
ATGTTGTACACTGCAACTCTTAAAAGAGCTGGGAATCAGGAAATAACTAG112120.10524462146667374No Hit
CTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCATCGACAGCACTG110840.10404311312313697No Hit
CTTTTAAGAGTTGCAGTGTACAACATGCATGTTGTATGTATGTGACTGGC110370.10360193427824455No Hit
CCTCTAGCCATCCTATCTTGCCTAAGGCAGGGAGGAGCCTGCACAACACT110330.10356438714250903No Hit
GTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACCTTGCTGC110030.1032827836244926No Hit
GCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTTGGACAATGTTAAATG109870.1031325950815505No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCT109550.10283221799566632No Hit
GAACACACAGGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTT108280.10164009643606343No Hit
GGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAGTTCTGTGAT108240.10160254930032792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAC56350.0102.765721
GGCTCAA136500.098.121631
TGAAGAC31550.087.283223
CAACACA68500.083.470874
ACTGACG35350.080.565974
CTGACGG35850.079.644465
CATGAAG34350.079.333491
TCCAACA73800.078.264862
GAAGGGT24500.075.1385961
AGCATTC217900.074.397325
TTCATCG36650.073.9451149
CCAACAC83750.073.203543
TCTCAAG141700.070.476482
AGCACAG183450.067.668755
GGGTAGT47200.067.255461
GTCCACT34200.064.847121
TGTTGTC65900.064.2205353
GCATTCA251900.064.1251456
ATTGACG59950.063.3361858
CAGCACA196050.063.208844
Produced by FastQC (version 0.11.5)