FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-38_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-38_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8281505
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACTACTGTGG852361.0292332130452135No Hit
CTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAA696730.8413084336723821No Hit
ATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACTACTGTGGGC395810.47794452819867883No Hit
TACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAA332370.4013400945842573No Hit
GCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAG315570.381053926792292No Hit
CCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAGTCCAGGA270420.3265348508513851No Hit
GCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTG268700.3244579336726839No Hit
GGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA265700.32083540370983293No Hit
TAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAATCCAC263570.31826340743620873No Hit
ATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGTCAA260340.3143631501762059No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATA260060.31402504737967313No Hit
TGGTAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTA259730.3136265690837596No Hit
TAGCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCT257430.3108492961122405No Hit
GGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGA248000.2994624769290123No Hit
AGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACA245180.2960572987639324No Hit
GAATTGATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCA240960.29096160661618875No Hit
CATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAATCC226940.2740323165897986No Hit
GGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTATGGTGA204440.2468633418684164No Hit
GGATACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACA203550.2457886579794373No Hit
GGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAAAAGAACAAT202420.24442417169343011No Hit
GTAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGT201410.24320458660593694No Hit
GTCCAGGATACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTT199520.24092239272934085No Hit
ATCACGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCT195110.23559727368394995No Hit
ACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAA193050.23310980310945897No Hit
ACCACCACCATAACCACCATCACCACCTCTACTACCACCTCCACCACCAT185890.22446403159812134No Hit
TAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTA185270.22371537540579883No Hit
CCTCTACTACCACCTCCACCACCATCACCATAACCACCATCACCACCTCT179530.21678426807687734No Hit
GATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGT178350.21535940629148928No Hit
CTACTACCACCTCCACCACCATCACCATAACCACCATCACCACCTCTACT177480.21430887260226253No Hit
ATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAAAA176290.2128719357169983No Hit
ATACATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAA157080.18967566885487602No Hit
CCCTAGAGTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGATA145010.175101023304339No Hit
GGTTATGGTGGTGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA141130.1704158845523851No Hit
CTTGATACATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTT140380.16951025206167236No Hit
TGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTT139360.16827859187430305No Hit
TCCCTAGAGTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGAT139000.16784388827876093No Hit
ATATCATTCCTTGGAATTTTCCACACAAAAAAGAACAATGACTGCTGATA134810.16278442143064575No Hit
TGGTAGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTG130290.15732647628661697No Hit
TCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAGTCCAGGATA129750.1566744208933038No Hit
GATCACGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCC121230.1463864357988071No Hit
GTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGA119250.14399556602332547No Hit
GGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTATGGTGG116700.14091641555490217No Hit
TAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATAACC113830.1374508618904414No Hit
GGCCAGAGCTAATCAATGGTCAACTTGCTGGAAAAGACAGCAAATTACAG112420.13574827280790144No Hit
ATTAGCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACT109930.13274157293873518No Hit
GCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACT107800.13016957666511098No Hit
ATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGTC106310.1283703867835617No Hit
GTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGATATCATTCC98540.11898803417977771No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCACCACCATA95790.115667381713831No Hit
ACCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCAT95500.11531720381742208No Hit
CGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCA93440.11282973324293108No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCAACACCATA91150.11006453537128819No Hit
CTACCACCACCTCCACCACCATCACCATAACCACCATCACCACCTCTACT90280.10901400168206142No Hit
TGGTAGTTATGGTGGTGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTG89660.10826534548973889No Hit
GGTGATGGTGGTTATGGTGGTGGTGGTGGAGGTGGTAGTAGAGGTGGTGA87790.10600730181289511No Hit
TCATAGGACTGATATCATTAACAATTGATAAATCTGAATACACAAGTTGC85290.10298852684385265No Hit
CACCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCA82930.10013880327307657No Hit
CCATCACCACCTCTACTACCACCTCCACCACCATCACCATAACCACCATC82850.10004220247406721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA79200.0129.407491
TTCCTAG107950.095.069042
CCTAGGT112400.091.560344
TCCTAGG115350.089.720853
CTAGGTA116750.088.210975
ATTAGCA37400.071.3200151
TAGGTAG149850.069.016456
ACGCACT51450.065.6426244
GGTTACG52900.064.398741
ATCACGC53300.063.3714941
TCACGCA53300.063.0941772
TAGCAAC98300.060.6850281
CGCACTA63800.053.276735
TTAGCAA53800.049.305722
GCACTAA100150.048.9973871
GTTACGG71400.047.6072542
TTACGGT71600.047.371333
AATAGAA296900.047.235582
TACGGTG77000.044.0494544
ACAGCCT153000.041.0215264
Produced by FastQC (version 0.11.5)