FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-37_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-37_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12694260
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA630400.4966024014003179No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA439730.34640065667474906No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA341930.2693579617874536No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG308360.24291293860374688No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC294790.23222306774873053No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA265590.2092205453488427No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT258490.20362746627215766No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA255460.20124056069436108No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT222430.17522092662352906No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT222400.17519729389503602No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG220020.17332243076792186No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT214940.16932062207643456No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC214690.16912368267232591No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC210220.16560240612686364No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC209770.1652479151994681No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG208280.1640741563509807No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC207940.16380631876139295No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG206520.16268770294605592No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC200210.15771695238635416No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT198850.1566456020280032No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA195470.15398298128445456No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC188010.14810630946585307No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC186740.14710585729298126No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA176800.13927554658562216No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC175670.13838538047905116No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG174470.13744007133932973No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA174000.13706982525960554No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC167100.13163429770620738No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT166290.1309962140368954No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA165400.13029510975826872No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC160080.1261042392388371No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC160040.1260727289341797No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC153310.12077112017557542No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG144960.11419334407834722No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA144840.11409881316437508No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC143640.11315350402465367No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA143130.11275174764027206No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT141810.1117119075865785No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG139900.11020729053918858No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA139480.10987643234028609No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG137240.10811185527947278No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA134470.10592976668194916No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT134160.10568556182085447No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA132840.1046457217671609No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA132750.10457482358168181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCG3650.077.4486145
TATGCGC23650.066.186385
TCGGACT451.19701E-464.415964
TGCGCTA24900.063.1518257
ATGCGCT24900.062.572246
TATCGGC1750.057.973913
CTCGTGG14400.057.3776971
CGCTAGT28700.054.0310869
ATCTCCC6250.053.348186145
TTCCGGA553.2266584E-452.706672
GCACTCG6550.052.0064661
GTTAGTC4250.051.153453
CTCCGAC2450.050.294983145
AAATTAG209100.049.2130555
CTTATCG2100.048.3180661
CTTCCGG1203.6379788E-948.318061
TCCGGAA604.958271E-448.3115923
GCGCTAG32550.047.640128
GAAGGAG9900.046.8479775
TTATCGG2500.046.3818662
Produced by FastQC (version 0.11.5)