FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_dDAP_LHY1_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_dDAP_LHY1_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20603745
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321130.15586001476915967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG141950.06.33723570
GTCGTGT157100.06.238554570
AGCGTCG191350.06.182873770
GAGCGTC208800.06.11877570
GCGTCGT176550.06.046920370
CGTCGTG164300.05.943876769
GTAGGGA103000.05.845118570
CGCGTCG7550.00785374555.563323567
GGGAAAG102950.05.507960370
AGAGCGT238200.05.422352369
CGTGTAG132750.05.35259869
TGTAGGG113900.05.347214770
AAGAGCG270700.05.1075370
Produced by FastQC (version 0.11.5)