FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_dDAP_HALO_GST_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_dDAP_HALO_GST_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934125
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105280.21337116510019508No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT2950.0045519819.49147235
CGCCTGT9700.09.38215169
GCCTGTG10700.09.1595770
CGGACTG5351.7429593E-69.1589196
CTTCGCC18450.08.72694669
TCGCCTG11500.08.52238270
TCTTCGC27500.08.27335270
GCGGACT4804.2871258E-48.0211135
GTCTTCG28750.07.67014469
GCTGGGG5250.00111805657.33299528
GAGTGTC42250.07.124581368
TTCGCCT17350.06.85930769
AAGAGTG68400.06.80606970
CCCTAGG5300.009899696.60380351
AGTGTCT44750.06.4921169
AGAGTGT56650.06.302329570
TTCGCCG9003.845779E-56.22269270
CCTGTGT12551.1701195E-76.13592170
GGGGGTA8609.085331E-45.697873665
AAAGAGT102150.05.653877370
Produced by FastQC (version 0.11.5)