FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_dDAP_CCA1_LHY1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_dDAP_CCA1_LHY1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407581
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80770.18325244618306505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4550.015.3844066
TACGGGC2356.8545993E-411.91459758
GGACTGT6855.456968E-1210.7295377
GACTGTA7901.0186341E-109.303468
CAGTCCG5109.417588E-68.92154927
CGACGTA4350.00144205388.045958553
ACTGTAG10951.0186341E-97.35133089
GCCGTGT5450.00165966787.064765570
ACAGTCC7602.4952284E-56.907879426
CAGAGCC15905.456968E-126.60428370
AGCCGTG8207.163976E-56.40293369
TGTAGGG72850.06.246192570
AGAGTGT37200.06.11606370
CTGTAGA14508.520146E-96.034263610
GTGTAGG78050.05.78519970
TACAGTC9203.463517E-45.706508625
AAGAGTG47350.05.618173670
AGCGTCG129350.05.601514370
GTAGGGA66350.05.59199170
CTACAGT10758.352603E-55.53468324
Produced by FastQC (version 0.11.5)