FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_HALO_LHY1_pGST_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_HALO_LHY1_pGST_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892094
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65150.16739061286803456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT5250.0090340496.666752361
GTGTAGG66300.06.651925670
GAGTGTT29850.06.56650270
GGGAAAG48600.06.55383570
CGTGTAG69400.06.40522869
GTAGGGA54800.06.195733567
TAGGGAA56200.06.16595668
AGTGTTA29700.05.892558670
TGTAGGG60500.05.843500666
AGAGTGT32400.05.83363369
GTCGTGT85600.05.806373670
AGGGAAA63500.05.67745769
CGTCGTG93800.05.48535769
GCGTCGT100900.05.411576370
TCGTGTA81650.05.358649768
AAGAGTG38350.05.20248768
TGGCATA13055.285181E-55.09585158
AGCGTCG108550.05.030198669