FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_HALO_LHY1_eGST_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_HALO_LHY1_eGST_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18505402
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG319370.17258203847719708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8500.012.3529756
CAGTCCG8756.7502697E-97.999675827
TGTAGGG297000.07.58967770
AGCCGTG17350.07.46442170
GTAGGGA265400.07.438272570
AGAGCCG27800.07.302592870
GCCGTGT13151.8189894E-117.18673970
CAGAGCC34900.07.02047570
GTGTAGG328800.06.994029570
CGTGTAG357600.06.969094870
AAGAGTG159000.06.846318770
GGGAAAG210400.06.787475670
GAGTGTT120450.06.6255570
GTCGTGT460850.06.607748570
TAGGGAA270450.06.31577670
AGAGTGT127300.06.29654269
GCGTCGT551750.06.293079470
CGTCGTG505450.06.22551670
TCGTGTA428700.06.180696569
AGGGAAA292300.06.04722970
Produced by FastQC (version 0.11.5)