FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_HALO_CCA1_eGST_LHY1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_HALO_CCA1_eGST_LHY1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485759
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107160.7212475239927876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4000.039.376426
GGACTGT4900.031.4286467
ACTGTAG4950.028.2828969
CAGTCCG3350.027.16424227
GACTGTA5300.027.0755378
CGGACTA2250.026.4453986
CACGGAC1551.2896635E-924.842954
ACAGTCC4250.022.23534826
CTGTAGA6350.022.04729710
TAGTCCG2108.367351E-1121.6652611
ACGGACT6550.020.841855
TACAGTC4600.020.54352825
CTACAGT4650.020.32194524
CCCGGAC1055.4613047E-420.0034164
TCGGACT3400.019.5608465
GGACTAG2951.8189894E-1218.9830977
AACGGAC2508.6401997E-1018.2031084
GTAGAAG8650.017.80231312
GGTCGCC1854.753065E-617.02649556
CCGGACT2401.9591607E-716.0433245
Produced by FastQC (version 0.11.5)