FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_HALO_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_HALO_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886494
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95950.1963575520608436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6000.019.2499666
GGACTGT9150.012.240297
GACTGTA10050.011.1436888
CAGTCCG7453.6379788E-1210.33512927
ACTGTAG12300.08.5361349
GTAGGAT33200.08.32949370
TAGGGTG13000.07.808477468
ACAGTCC8654.651156E-87.68761126
GGATAGG18100.07.15570970
CCGGACT6401.6034006E-47.1094355
AGAGTGT58100.06.808192770
GAGTGTA49500.06.718122570
AGTGTAG44350.06.70895469
AGGGTGT13703.4742698E-106.643275769
AGGATAG26050.06.584425470
TCGGACT8005.095452E-56.5625565
GGGTGTA11651.8760147E-76.30990670
GTGTAGG158600.06.290297570
GGGGGTA6200.00641000556.210302467
CTACAGT12409.26284E-86.20947624
Produced by FastQC (version 0.11.5)