FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL8_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL8_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688910
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67710.1835501543816466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5950.025.2935056
GGACTGT8400.017.4993387
GACTGTA8250.016.1206048
CAGTCCG5100.014.41102527
ACTGTAG9450.012.5921169
GTACGTC15200.011.283998570
ACGTCCG2802.3446519E-411.25110170
TCGGACT5107.010385E-910.9805735
AACGGAC6251.3278623E-1010.6407534
CTGTAGA12350.09.9185210
ACAGTCC7201.7553248E-99.23563826
TGTACGT21900.09.1104869
ACGGACT13100.08.8169395
TCCCGAC3200.0088846378.74943215
GTGTACG21750.08.69026968
CGGACTA4057.103861E-48.6417676
CCGGACT4502.1375496E-48.55569655
GTCGTGG11001.6370905E-117.955323770
CTACAGT9252.2573658E-97.945537624
TACAGTC8858.3273335E-97.909198325
Produced by FastQC (version 0.11.5)