FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL7_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL7_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096826
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60040.1465524774544977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGC19750.06.91422461
GTGTAGG78750.06.62267270
CGTGTAG81550.06.438283469
GTAGGGA63400.06.348778768
TGTAGGG71150.06.34617970
TAGGGAA65050.06.241852369
AGAGTGT35300.06.147725670
AGGGAAA70650.06.04428870
CACGTAG6450.0096652975.968668520
GGGAAAG55600.05.9177469
GTACTAG26800.05.87927441
GCAGACA8000.00239013415.687330726
AAGAGTG41550.05.64425469
TCGTGTA96350.05.594362768
GAGTGTT33850.05.58383570
GTCGTGT100350.05.37163169
TACTGCA19601.378794E-95.3585532
CGTCGTG107100.05.098198468
GGAAAGA68150.05.033356770
Produced by FastQC (version 0.11.5)