FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL6_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL6_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141479
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109570.17840979347157257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG12150.08.93077670
CGGACTG9051.8189894E-118.8948176
CGCCTGT10353.6379788E-118.1166170
CTTCGCC23050.07.441019569
GTCTTCG32850.07.24564970
GGACTGT10452.3861503E-87.03326947
GAGTGTC48000.06.78186368
AGAGTGT66850.06.70212270
TCTTCGC31000.06.436012770
AGTGTCT51400.06.401356769
CCGACTG6900.00289384426.08646618
TTCGCCT20300.06.034979370
AAGAGTG78900.05.85604369
TGTAGGG131650.05.822734470
GTAGGGA118650.05.782186570
GCGGACT7350.00584005055.7143835
TAGGGAA120900.05.558769770
GTGTAGG143350.05.46962669
CGTGTAG151650.05.44720170
GGGAAAG106950.05.367435570
Produced by FastQC (version 0.11.5)