FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL5_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL5_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18447165
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348940.1891564367749733No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC5401.7710707E-47.778229768
GCCGTGT40150.07.410324670
GTTCAGA155850.06.76026270
AGCCGTG54350.06.633481569
CAGAGCC98550.06.60632870
AGAGTGT247700.06.59925270
GAGTGTT223500.06.577723570
GTGTTCA169350.06.510722670
ACGGATC97100.06.38260561
TGTTCAG167500.06.373659669
CCCCTGG25150.06.262500339
TCAGAGC123650.06.199459670
TAGCCGC19200.06.197940355
ACGGTCG46150.06.14550071
ACCCCTG26000.06.057715438
AGTGTTC187900.05.88659569
TAGGGAA449150.05.8838270
GTAGGGA458450.05.863717670
AAGAGTG288900.05.82775670
CCGTCCC31600.05.759968370
Produced by FastQC (version 0.11.5)