FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL4_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL4_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17640642
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287810.16315165853941144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC11607.2759576E-127.845525369
GATAGGG54700.07.230990470
CGCCCGA11101.0333679E-86.93704254
AGTGTAG138700.06.66245270
GGATAGG70350.06.517991570
GTGTAGG500600.06.439844670
GTAGGAT116650.06.3014670
CGTCCCC17309.094947E-126.27203868
GCACGCC23500.06.255573763
TAGGGAA341550.05.94402170
GTAGGGA341450.05.9355170
TGTAGGA138100.05.85497469
GAGTGTA176050.05.686390470
CCCTGCG27900.05.645262760
GCCCGAT13151.8005467E-65.58935955
AGGGAAA371650.05.528541670
GCCCCGC7600.0084543845.52621178
AGAGTGT195550.05.42364770
TGTAGGG389300.05.38578669
CGTGTAG446550.05.361578569
Produced by FastQC (version 0.11.5)