FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL3_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL3_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9269903
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200540.21633451827920963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG16400.011.09828670
TGTGCCG15900.011.00700970
CGGACTG16550.010.1511736
GGACTGT18100.09.6683587
AGAGCGG27450.09.3084470
CCCTGCG10700.09.15908153
GCGGACT10709.094947E-128.1779285
TCGTGTT51800.08.10863870
CGGAAGC39700.07.935027670
GTCGTGG51100.07.877227370
GTAGGGG18700.07.674298370
GACTGTA19050.07.34896668
CGGAAGG21450.07.343137770
GGTCCCT15350.07.068637450
GTGTGCC36900.07.019428769
CTAACGC12301.6043487E-96.82960464
ACTGTAG21800.06.5823959
AGTGTGC42250.06.46185768
CGGACTA13106.2336767E-96.41228686
CCGACTG16601.2187229E-106.11383518
Produced by FastQC (version 0.11.5)