FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL2_Col_B_j2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL2_Col_B_j2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028492
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74480.18488307783657013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCG3803.7590944E-49.21085770
TGTAGGC22000.08.27302569
GTAGGCT17150.08.16355970
AGTGTAG32300.07.80213970
GAGTGTA34350.07.744092569
TGTAGGG88150.07.107459570
GTGTAGG112300.06.950383769
TAGGCTA18250.06.712570770
GGCTATA16251.0913936E-116.46177170
AGAGTGT42750.06.386115668
CGTGTAG99850.06.344666568
GTAGGGA78750.06.311338469
TAGGGAA78100.06.27423470
GGGAAAG66100.05.8776769
AGGCTAT15657.4287527E-95.814905670
AAGAGTG51600.05.56221670
TCGTGTA118450.05.525737370
GTCGTGT125650.05.48766370
GCTATAG13452.7206497E-65.46488169
GGAAAGA77550.05.416055770
Produced by FastQC (version 0.11.5)