FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL2_Col_B_j1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL2_Col_B_j1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454088
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86950.19521392482591274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4351.8189894E-1214.48263556
GTAGGCG4153.916284E-912.65134770
AGGCTAT19800.09.72279470
TGTAGGC28250.09.66439469
TAGGCTA22250.08.96692169
GTAGGCT23600.08.75051570
GGACTGT6806.345181E-88.7497297
AGTGTAG45350.07.48665970
TCGGACT8101.0421263E-67.34569845
GAGTGTA48900.07.229464570
AGAGTGT57900.06.710239470
CAGTCCG6050.00497789456.363082427
GTAGGGA111000.06.24361170
GTGTAGG160300.06.113897370
GGGAAAG89650.06.01269169
TAGGGAA107700.05.947425470
AAGAGTG64850.05.937193469
AGGGAAA107950.05.93365270
TGTAGGG124200.05.918222470
CGTGTAG143500.05.878394670
Produced by FastQC (version 0.11.5)