FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9173073
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147390.1606767982768697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCG9750.011.48718853
GTAGGCG8750.010.00098970
CTAACGC12900.09.76787664
CCGTCCC13250.09.24619870
GTCCCCC5906.830742E-78.89918569
GGACTGT17750.08.478517
TGTAGGC41650.08.3201569
GCTAACG15300.08.2356663
CGGACTG21950.07.33473836
CGTCCCC7401.7171964E-57.09502668
GCGGATG18450.07.01889957
GTAGGCT34900.06.920455570
TCGGACT12301.6061676E-96.8291625
CCGACTG17050.06.773607718
TAACGCA17150.06.734992565
AGTGTAG67750.06.66486670
GCACGCC11107.5424396E-86.62162759
CCTGCGG18100.06.57448354
TCCCTGC17301.8189894E-126.473993352
GTGTAGG213300.06.3836670
Produced by FastQC (version 0.11.5)