FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL16_Col_B_j2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL16_Col_B_j2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640109
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66410.18243959178145489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG3051.9572326E-713.77162270
CGGACTG3750.00341082958.3999976
GTGCCTC14100.07.4474270
GTGTGCC14900.07.28247770
AGTGTGC18050.06.59333970
GAGTGTG28500.06.14085570
AAGAGTG39850.06.060723370
TGTGCCT18001.5279511E-105.833891469
GACTGTA7200.00464631945.8332518
CGTGTAG67100.05.477349870
GTAGGGA53800.05.46513270
TAGGGAA55450.05.42883469
TGTAGGG59800.05.385057470
GTCGTGT82350.05.31312670
GGGAAAG50550.05.26262169
TCGTGTA78150.05.195635369
AGAGTGT33200.05.166157269
GTGTAGG64550.05.15125668
GCCTCTA13004.954777E-55.11580470
CGTCGTG88450.05.065493669
Produced by FastQC (version 0.11.5)