FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL16_Col_B_j1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL16_Col_B_j1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033644
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29740.2877199500021284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2550.045.2962466
GGACTGT3200.032.814047
GACTGTA3350.030.299938
ACTGTAG3800.026.7091969
GCGGACT1802.8558134E-1023.334435
TCGGACT2550.023.3344295
CTGTAGA4750.022.10416210
TGTGCCG1356.0187213E-620.7427270
TAGTCCG1356.0262864E-620.73970811
GTGCCTG1408.243125E-620.0019170
AACGGAC1651.5921178E-619.0918064
CAGTCCG2203.5051926E-919.08903527
CACGGAC1301.0571238E-418.847044
CGGACTA1702.1184042E-618.5302836
GACACCC1351.412806E-418.14724511
CCGGACT1401.8647764E-417.5008245
GTAGAAG7050.015.88573512
ACGGACT3553.765308E-1014.7894285
CTAGTCC2151.9759877E-514.65043410
ACAGTCC2901.056942E-714.48133826
Produced by FastQC (version 0.11.5)