FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL15_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL15_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350215
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95460.15032561889636809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCC22250.07.70861270
GTCTTCG31750.07.27629570
CGCCTGT10703.7414793E-86.869824469
GTAGGGA127400.06.70394670
AGAGTGT68950.06.59962470
TCTTCGC29200.06.4732370
TAGGGAA124050.06.37707970
TCGCCTG13451.0928488E-86.245958370
TGTAGGG138800.06.153333769
GAGTGTC48950.06.149727369
GCCTGTG13356.1510946E-86.030546769
CCTGTGT14851.4364559E-85.89282770
GTACGGG8457.232236E-45.80088661
AGTGTCT51450.05.78269168
GTGTAGG150350.05.75029368
AAGAGTG82300.05.614152469
TCGTGTA185650.05.486661470
GTCGTGT194000.05.430938269
CGTGTAG158400.05.36966967
GGGAAAG109750.05.294362570
Produced by FastQC (version 0.11.5)