FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL14_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL14_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12010363
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185660.15458317121639037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC8350.011.3180369
CCGTCCC17900.09.7770770
CGTCCCC11203.6379788E-128.12547868
AGCCGTG24600.08.11019970
TCCCCCG12554.5474735E-117.251392470
GCACGCC17700.07.11858859
GCCGTGT19400.06.85604570
CAGAGCC48050.06.483211569
TGTTCAG83800.06.474100670
CCCTGCG14451.200533E-96.297580753
GGACTGT20300.06.2068747
GTGTTCA87250.06.17798870
GCGTGCG10802.2053282E-66.15743641
GGTACGC16153.8926373E-106.0705411
TCCGTCC33450.05.96447669
ACCGCGT13152.9184775E-75.855394438
GAGTGTT120000.05.833651570
CCCAGTC19809.094947E-125.83348264
GTGTAGG271500.05.76274670
TAGGGAA228150.05.707083770
Produced by FastQC (version 0.11.5)