FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL13_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL13_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8053321
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144080.17890755875743683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG10900.024.0822536
GGACTGT13900.017.6256667
TGTGCCG10650.013.14716270
GACTGTA17850.012.7448528
CGCGGAC2902.4297997E-512.0696314
CAGTCCG10750.011.72077627
ACTGTAG21900.011.1869969
GCGTCGG7302.2191671E-109.59022570
GCGGACT7756.91216E-119.4839225
TCGGACT12950.08.9189675
ACAGTCC15400.08.40897926
CTGTAGA30450.08.04577110
GTGTGCC40800.07.978925770
TACAGTC17650.07.93190625
GTGCCTC33050.07.09618670
CTACAGT19800.07.07039424
CCGGACT10201.13872375E-76.86278255
TCGTGTT39000.06.64184570
TGTGCCT40500.06.482321369
AGTGTGC48800.06.455754869
Produced by FastQC (version 0.11.5)