FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL13_C24_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL13_C24_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288734
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15200.11794520824312853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4250.059.2927676
GGACTGT4950.050.9059567
GACTGTA6050.039.913358
ACTGTAG6250.039.1960689
CCGGACT1900.038.6863335
CTGTAGA6800.036.02579510
CAGTCCG3950.032.7827927
TCGGACT2850.031.9315765
GCGGACT1900.031.3175075
ACAGTCC4650.028.6003926
CTACAGT5300.025.75213224
ACCGGAC1701.4006218E-1024.7110734
GTAGAAG9850.024.51530612
TACAGTC5600.024.37255325
ACGGACT5500.022.9103495
AACGGAC2401.8189894E-1221.8795994
TCTACAG6450.021.70324923
ATCGGAC2453.092282E-1120.0042024
AGTCCGA2504.1836756E-1119.59879528
CGGACTC1801.7036473E-719.4440026
Produced by FastQC (version 0.11.5)