FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL12_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL12_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7633619
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98980.12966326980688977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC5400.012.96424169
CCGTCCC12400.010.44457870
CGTCCCC7251.9463187E-109.65580868
TCCCCCG7302.2191671E-109.58998670
CCCTGCG10350.09.13043753
CTAACGC11000.08.59147564
TCGGACT10001.6370905E-118.4001675
GCACGCC9051.4752004E-98.12128459
GTAGGCG5804.7322163E-57.845600670
CGGTCGC6704.2257634E-67.83566957
TGTAGGC35550.07.77854470
GACCAGC14350.07.56112661
CACGGGA15300.07.54897254
GGCCCCG4650.00277961027.52663729
ACGGGAG15300.07.320071755
AGTGTAG55400.07.13969370
CACACGG15700.07.133853452
ACACGGG15750.07.111113553
TAGGCTA26650.06.961286570
GCGTGCG15201.8189894E-126.908122541
Produced by FastQC (version 0.11.5)