FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL11_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL11_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278602
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43230.13185497965291304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3450.026.3762026
GGACTGT5200.018.1726577
GACGGAC3051.9577237E-713.7712244
TAGTCCG3658.85484E-913.42414311
CAGTCCG3851.8686478E-812.72678427
GACTGTA7350.011.9044888
CGGACTA4351.239132E-610.4595286
TCGGACT5703.5579433E-910.4385145
ACTGTAG8600.09.7670689
CCGCCTG3000.00522755559.3332654
CTGTAGA10650.08.87289910
TACAGTC6959.0221874E-88.560822525
ACAGTCC6251.5658534E-68.39967726
CTACAGT7857.436029E-88.02504724
CTAGTCC5703.813183E-57.982149610
ACTAGTC6805.2121995E-67.7202929
ACTGCCT4750.00342159657.368138317
GGACTAG5904.9108156E-47.11847977
AGAGTGT30700.06.270787770
CGTGTAG67600.06.26522170
Produced by FastQC (version 0.11.5)