FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_ColSPL10_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_ColSPL10_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525479
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45910.18178729658809278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3600.020.4154426
CAGTCCG2553.9090264E-715.09713527
GGACTGT4851.8189894E-1213.7102457
GACTGTA5701.8189894E-1212.2797228
ACTGTAG6351.4551915E-1111.0227429
ACAGTCC3955.166451E-59.74644426
GTGTCAG16350.08.563024570
AACGGAC3700.00301659158.5146894
GTGTGTC17800.08.06211368
CTGTAGA9151.8244464E-98.032145510
TACAGTC4804.2893522E-48.02035225
TGTCAGT13100.08.0155870
CTACAGT5956.5091655E-57.646600224
GTACGTG6452.1466296E-57.597195670
AGAGTGT29700.07.188832870
GTCAGTA10552.8538125E-86.96709769
ACGGACT8854.1864187E-66.72355945
CAGTACG8351.2790311E-56.70684870
CCAACAG6350.00113458656.613776724
AAGAGTG38200.06.597116570
Produced by FastQC (version 0.11.5)