FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n02_C24SPL11_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n02_C24SPL11_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515808
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7620.14772938767913643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1900.051.5808756
GGACTGT2200.046.1380927
TCGGACT1600.045.939225
CGGACTA1200.043.7516376
TAGTCCG1000.041.99749811
GCGGACT654.8617403E-737.6937185
CCGGACT1250.036.401365
GACTGTA2650.035.6617138
ACCGGAC806.2376785E-835.001314
ACTGTAG2750.034.364929
CTGTAGA3050.032.13234710
GGACTAG1551.8189894E-1229.3559367
CGGACTC853.95228E-628.8246066
AGCGGAC500.007205425328.0010474
CAGTCCG2050.027.31809627
AACGGAC906.165119E-627.223244
ACGGACT2550.026.0794075
TACAGTC2450.024.28662325
CTACAGT2650.023.77447524
CTAGTCC1802.8558134E-1023.33420610
Produced by FastQC (version 0.11.5)