FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18152643
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639220.35213604983031943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT50150.021.3193234
GCTTGAA77700.014.06984716
ATGCGCA13600.013.64106436
TGCGCAT14050.013.20518337
GCCCGAC5153.6379788E-1212.9035021
AGGGGGG67800.012.2258731
GCCCTGA9500.012.1493091
GCCGTCT92200.012.0881846
AATGCGC16200.011.88419435
TAATGCG17800.011.40506334
CTCGTAT41150.011.14856744
GGGCCAC7900.011.0681291
GCGCTCC3501.3730098E-510.9922321
GCGCATC14600.010.79291838
TGCCGTC105850.010.5627255
TATGCCG106850.010.0051383
CTTGAAA116150.09.65290217
GACTGTA18700.09.5239978
AACGGGG9600.09.11599429
ACGGGGG10800.09.07505331
Produced by FastQC (version 0.11.5)