FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_dDAP_LHY1_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_dDAP_LHY1_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20603745
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGC44150.041.0643335
TGCGCAT44350.039.61646737
ATGCGCA48100.037.692136
GCGCATC44550.037.00316238
TAATGCG60750.030.07390834
TGCCGGC9650.029.74076850
TGCCGTC17800.028.90433750
CATCGCG18150.027.76844441
GTATGCC25450.025.85447747
CGCATCG31150.024.15717339
ATGCCGG12050.023.23638749
CAGTCAC80000.023.1874327
GCCGTCT15400.023.18174751
GCATCGC22500.023.1776540
TATGCCG27900.023.08231248
CGCATCT40700.022.35859939
CTAATGC83950.021.9712933
CGTATGC28600.021.41596646
ATGCCGT23400.021.239249
CCAGTCA91150.020.35100726
Produced by FastQC (version 0.11.5)