FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_dDAP_HALO_GST_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_dDAP_HALO_GST_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934125
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT51760.10490208496947281TruSeq Adapter, Index 3 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC23950.046.76400438
TGCGCAT27350.043.25403237
ATGCGCA30700.040.2442336
TGCCGTC9650.039.89629750
AATGCGC31650.039.36801535
TATGCCG10800.038.88881748
GTATGCC12150.036.8723647
ATGCCGT10300.036.6989649
TAATGCG37450.033.45788234
CATCGCG9150.033.27862541
CGCATCG14550.033.19581239
GCCGTCT8500.032.94111651
CGTATGC12450.032.61038246
CGCATCT22250.028.62916239
CTGCGTG5300.028.39617359
GCATCGC11850.026.87758840
TGCCGGC6050.026.0330150
CTAATGC48950.025.74050133
CGCGTAT6400.024.6093344
CATCTCG19900.024.4471941
Produced by FastQC (version 0.11.5)