FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_dDAP_CCA1_LHY1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_dDAP_CCA1_LHY1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407581
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC17500.043.1998438
TGCGCAT19400.040.59263237
ATGCGCA20550.039.6835536
AATGCGC22100.037.21705635
CGTATGC8600.035.40684546
TATGCCG8500.034.17634248
TGCCGTC7400.033.10798650
TAATGCG25350.032.85983734
GTATGCC9850.032.33490447
ATGCCGT7850.029.872549
CATCGCG7750.029.80634141
CGCATCG11800.028.77107839
GCCGTCT6350.028.66131251
CGCATCT17550.024.9286839
TGCCGGC3550.024.64779550
ATCGCGT7700.023.63627642
GCATCGC10300.023.44651440
CTAATGC36100.023.36556233
CGCGTAT6250.021.83991844
CTCGTAT12250.021.42849344
Produced by FastQC (version 0.11.5)