FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_HALO_LHY1_pGST_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_HALO_LHY1_pGST_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892094
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT51950.13347570742124934TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10200.047.35283750
GCCGTCT10350.045.9902451
TATGCCG10750.044.93013448
ATGCCGT11150.043.3182949
GTATGCC11150.042.6904947
CGTATGC11600.041.93956446
CTCGTAT14100.033.51056744
CCGTCTT14700.033.09516552
TCGTATG15450.031.03553245
CTGCTTG16200.027.43821159
GCTATCT16150.027.3064439
TCTCGTA16850.027.00290943
AGCTATC18700.025.08015838
CGTCTTC20400.024.5342653
TATCTCG19450.023.21331641
ATCTCGT20450.022.93393342
CTTCTGC22500.021.4666256
CCTGAAG24300.020.4526333
CTGAAGC25500.020.03917334
CACCTGA25500.019.49015431
Produced by FastQC (version 0.11.5)