FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_HALO_LHY1_eGST_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_HALO_LHY1_eGST_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18505402
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT23650.034.77783251
TGCCGTC24850.033.80264350
ATGCCGT27800.030.97106649
CGTATGC30200.028.74157546
TATGCCG32750.027.03802948
GTATGCC31550.026.95707347
CTCGTAT52350.015.91204844
TCGTATG54700.015.67633745
CTGCTTG53550.015.55547659
GCTATCT55550.014.80640539
CCGTCTT59200.014.42560352
CGTCTTC59250.014.29528653
AGCTATC60600.013.80356138
CGGACTG8400.013.7500426
CTTCTGC69150.012.19805756
CCTGAAG73850.012.1326433
CTGAAGC81400.011.35129434
CACCTGA83150.010.77565231
TCTCGTA81950.010.42093543
GTCACCT88200.010.07931329
Produced by FastQC (version 0.11.5)