FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_HALO_CCA1_eGST_LHY1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_HALO_CCA1_eGST_LHY1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485759
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT29470.1983497996646832TruSeq Adapter, Index 19 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3650.045.0681276
GGACTGT4350.036.20667
ACTGTAG5200.032.9805039
CGCGGAA3200.032.8166471
GACTGTA4950.031.817928
CAGTCCG3250.027.99977327
CTGCGCT500.007211324327.99977137
CCGGACT2250.026.444235
GCGGACT1900.025.7892635
ACAGTCC3700.024.59439326
CTGTAGA6750.024.3701710
TAGTCCG2051.8189894E-1223.90224511
TGCCGTC12350.023.52207650
ATGCCGT12850.023.15156249
CGGACTA2350.022.3402446
GCCGTCT12400.021.73442551
TATGCCG13400.021.67892648
TCGGACT2750.021.6361875
GTATGCC13850.020.97455847
CGTATGC14000.020.49983246
Produced by FastQC (version 0.11.5)