FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_HALO_CCA1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_HALO_CCA1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886494
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT129940.26591662652200126TruSeq Adapter, Index 19 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18900.050.555248
TGCCGTC19000.050.28911650
GCCGTCT19300.048.78204351
ATGCCGT19750.048.3794149
GTATGCC21700.044.35452747
CGTATGC22000.044.0678746
CTCGTAT25100.035.97584544
CCGTCTT26650.035.59074452
TCGTATG27900.034.2470745
GCTATCT30200.030.36402739
TCTCGTA30050.030.16617843
TATCTCG30450.029.76990741
CTGCTTG29900.029.14695259
CTTCTGC32300.028.0648256
CGTCTTC33700.027.83363253
ATCTCGT33950.026.90702642
AGCTATC36350.025.5156438
TTCTGCT40300.022.66733657
CTATCTC41100.022.56675140
TCTGCTT40950.021.7092558
Produced by FastQC (version 0.11.5)