FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL8_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL8_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688910
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8350.034.79024548
ATGCCGT8400.033.33316449
GCCGTCT7500.033.13316351
TGCCGTC8750.031.99983850
GTATGCC9850.029.49223547
CGTATGC9950.028.14056246
CGGACTG5650.025.39816
TCGTATG12800.020.78114545
GGACTGT7350.019.9998997
CCGTCTT13700.018.90501452
CTCGTAT12750.017.84304844
GACTGTA9100.015.7693648
TCTCGTA15250.015.14746543
ACTGTAG9600.014.5834579
CTGCTTG14600.014.38348959
AATCTCG19950.013.50870341
CGTCTTC19350.013.20406953
CAGTCCG5051.8189894E-1213.16825227
CCGGACT6200.012.9838065
ATCTCGT19550.012.71093442
Produced by FastQC (version 0.11.5)