FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL7_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL7_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096826
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6800.031.39697648
ATGCCGT7700.027.727249
TGCCGTC6950.027.69776750
GTATGCC7800.027.37172547
GCCGTCT7050.024.32617851
CGTATGC8250.024.18175746
TCGTATG13050.013.67812545
CTCGTAT12450.013.21281744
CCGTCTT13650.012.5640752
TGCGCCG2000.002783565812.24996835
CTGCTTG16850.010.17801659
AATCTCG17200.010.17439241
CGTCTTC17450.09.82805453
CATTCAG27850.09.80248833
CTTCTGC20300.08.96549356
TCTCGTA17800.08.65166343
CAGTCAC32850.08.41712927
ATCTCGT19200.08.38539442
GTCACAT32150.08.27369529
CACATTC35250.08.04253231
Produced by FastQC (version 0.11.5)