FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL6_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL6_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141479
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT63750.10380235770569271TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15800.035.66437548
GTATGCC16400.034.3595847
GCCGTCT13350.033.2957151
TGCCGTC15350.033.06171850
CGTATGC17600.031.81801846
ATGCCGT16800.031.4581749
CTCGTAT22300.022.44383244
TCGTATG24500.022.285645
CCGTCTT25000.019.03990252
TCTCGTA29900.017.32432643
CTGCTTG24700.016.72056259
AATCTCG33800.015.53246541
ATCTCGT33050.015.46134342
CATTCAG49200.014.72553433
AGGGGGG9200.014.077391
CGTCTTC33600.013.854094553
ATCGCGT6600.012.72720742
CTGCGTG5551.4551915E-1111.98192159
CTTCTGC35950.011.68283456
AATCGCG7500.011.66660741
Produced by FastQC (version 0.11.5)