FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL5_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL5_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18447165
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT399600.21661865115859263TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG75650.045.06255748
TGCCGTC74400.043.98498550
GCCGTCT73550.040.30570651
GTATGCC86000.040.24978647
ATGCCGT87150.037.50984649
CTCGTAT108100.028.39485544
CGTATGC121950.028.26963646
AATCTCG128750.025.60763541
TCGTATG130750.025.37654145
CCGTCTT130200.023.76331952
CTGCTTG110600.023.44924759
ATCTCGT149850.021.93181842
CTTCTGC124800.021.90293356
TCTCGTA151200.021.20359443
CTGCGTG18050.020.74781459
CATTCAG224100.019.94411833
GCTTGAA148150.019.70306261
CGTCTTC159300.018.9390153
TGCTTGA142100.018.8177960
TCAGAAA241300.018.69654736
Produced by FastQC (version 0.11.5)