FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL4_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL4_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17640642
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT231520.13124238902416363TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51300.040.25313648
TGCCGTC51200.038.21262750
GTATGCC60100.035.11623447
GCCGTCT50950.034.6221451
ATGCCGT67200.029.99979649
CGTATGC100900.021.22879646
CTCGTAT91100.021.2073244
AATCTCG101600.019.80793641
TCGTATG112450.018.36359245
ATCTCGT115500.017.36351842
CTGCGTG15300.016.24171859
CTGCTTG99100.015.92824759
CTTCTGC111350.015.24470756
CCGTCTT127500.015.20782752
TCTCGTA129650.015.03653443
CATTCAG176750.014.87118633
CACATTC182750.014.32548331
TCAGAAA186700.014.32234536
GCTTGAA129100.013.9891861
TCTGCTT119900.013.60290958
Produced by FastQC (version 0.11.5)