FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL3_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL3_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9269903
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18250.030.49299648
TGCCGTC19000.029.47353650
GCCGTCT19300.026.4765551
GTATGCC22500.025.82209247
ATGCCGT21200.025.75458549
CGTATGC25350.022.09061646
TCGTATG33000.016.8635545
CTCGTAT31350.016.5230444
CCGTCTT38600.013.78231352
TCTCGTA41650.012.77304543
CTGCTTG35850.012.59407859
AATCTCG46200.012.1211541
GGACTGT14750.011.8646677
ATCTCGT46900.011.34322642
CATTCAG70650.011.096933
CGTCTTC52600.09.78131953
GTCACAT84300.09.75677129
GCTTGAA58450.09.5209161
GACTGTA19150.09.3213028
CACATTC85900.09.24908131
Produced by FastQC (version 0.11.5)