FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL2_Col_B_j2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL2_Col_B_j2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028492
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC19850.042.3171538
TGCGCAT22000.039.931637
ATGCGCA23800.037.49979436
AATGCGC24950.036.61302635
TATGCCG8150.036.5028748
TGCCGTC8050.033.47807750
TAATGCG28250.032.70778334
GTATGCC9400.032.021147
CGCATCG13200.031.81800739
ATGCCGT8600.030.93006149
CATCGCG8200.030.73153941
GCCGTCT7350.029.99983451
CGTATGC8650.029.94203246
ATCGCGT6550.028.32045642
CTGCGTG4000.027.99984759
GCATCGC9550.027.4867640
CGCATCT16600.026.35527639
CTAATGC36650.025.11582433
TGCCGGC3850.024.54531950
CGCGTAT5000.024.49986544
Produced by FastQC (version 0.11.5)