FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL2_Col_B_j1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL2_Col_B_j1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454088
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT112570.25273411751182284TruSeq Adapter, Index 19 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17450.050.7447148
TGCCGTC18050.048.86400250
ATGCCGT18300.048.57896849
GCCGTCT17550.048.46127751
CGTATGC18750.047.78640746
GTATGCC19750.045.01241347
CCGTCTT22300.037.98185752
CTCGTAT21600.037.4303544
TCGTATG24750.036.20182445
CTGCTTG24750.033.37355859
GCTATCT24650.032.6570239
TATCTCG26550.030.5836441
CGTCTTC28250.030.10602853
CTTCTGC28700.029.02423356
TCTCGTA28150.028.59664743
ATCTCGT29950.027.46228642
AGCTATC31550.026.29145838
CTATCTC32800.024.75596440
TCTGCTT34700.023.60217758
TTCTGCT33800.023.4022457
Produced by FastQC (version 0.11.5)