FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL1_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL1_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9173073
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18850.035.64966248
GTATGCC22200.031.21603447
TGCCGTC21350.030.16376150
GCCGTCT20550.029.97549651
ATGCCGT24300.027.22206749
CGTATGC33600.021.24987846
CTCGTAT34300.018.57132144
TCGTATG41400.017.24627945
CCGTCTT42600.015.93887452
CTGCGTG7100.015.77455759
AATCTCG45600.014.81351141
CTGCTTG37800.014.35176959
ATCTCGT48000.013.92700442
TCTCGTA47950.013.35758743
CGCGGAC2203.9332508E-412.7274774
CGTCTTC54900.012.11286353
CATTCAG71100.012.0604133
CTTCTGC50700.011.94273256
TGCGTGA10150.011.72432660
CCCTGCG9650.010.51807453
Produced by FastQC (version 0.11.5)