FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL16_Col_B_j2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL16_Col_B_j2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640109
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC12150.031.68702538
TGCGCAT13350.029.88743437
ATGCGCA14850.028.28263336
AATGCGC16550.025.5889535
TGCCGTC5250.023.33317250
TATGCCG5300.023.11304948
CATCGCG4600.022.06506741
TAATGCG19850.021.8638334
ATGCCGT5800.021.12054449
CGTATGC6800.020.58809546
GTATGCC7300.020.13684847
CGCATCT12850.018.24890139
CGCATCG9100.016.5383539
GCATCGC6350.016.5353240
GCCGTCT5300.016.50954651
CTAATGC27750.016.14403333
ATGCCGG2906.2354957E-915.68954849
GCGTGAA5700.015.35077261
CATCTCG14250.014.98235341
ATCGCGG3806.730261E-1114.73674142
Produced by FastQC (version 0.11.5)